Effect of propolis, alcohol and dexamethasone on mast cells in surgical skin wounds

  • Túlio Garcia Rocha Universidade Federal dos Vales Jequitinhonha e Mucuri
  • Poliana Ribeiro Barroso Universidade Federal dos Vales Jequitinhonha e Mucuri
  • Ricardo Lopes Rocha Universidade Federal dos Vales Jequitinhonha e Mucuri
  • João Luiz de Miranda Universidade Federal dos Vales Jequitinhonha e Mucuri
  • Sandra Aparecida Marinho State University of Paraíba
  • Flaviana Dornela Verli Universidade Federal dos Vales Jequitinhonha e Mucuri
Keywords: anti-inflammatory, healing, mast cells, surgical wounds, propolis

Abstract

Background: The present study determines the anti-inflammatory effect of the topical application of ethanolic extract of commercial propolis, used in wound care, in the mast cell population in connective tissue during the initial healing process of surgical skin wounds. Method: Sixty Wistar rats were divided into three experimental groups: Group 1 (n = 20) – 30% ethanolic extract of propolis; Group 2 (n = 20) – 70% alcohol; and Group 3 (n = 20) dexamethasone. Each substance was applied topically every 12 hours. The specimens were submitted to histological analysis with Toluidine Blue stain. Mast cell counts were performed using the Axio Vision® software program. Statistical analysis involved the Kruskal-Wallis and Mann-Whitney tests. Results: Significantly smaller numbers of mast cells were found in the groups submitted to the propolis extract and 70% alcohol in comparison to dexamethasone (p < 0.0001). Moreover, the reduction in the number of mast cells was maintained in all healing phases in the propolis extract group. Conclusion: The present findings demonstrate a possible anti-inflammatory property of propolis extract and 70% alcohol, which was more effective at reducing the mast cell population in surgical skin wounds at different postoperative times in comparison to the standard pharmacological treatment, dexamethasone.

Author Biographies

Túlio Garcia Rocha, Universidade Federal dos Vales Jequitinhonha e Mucuri
Undergraduate Student Pharmacy Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM) Diamantina Minas Gerais Brazil
Poliana Ribeiro Barroso, Universidade Federal dos Vales Jequitinhonha e Mucuri
MSc Science and Technology Institute (ICT) Universidade Federal dos Vales Jequitinhonha e Mucuri Diamantina Minas Gerais Brazi
Ricardo Lopes Rocha, Universidade Federal dos Vales Jequitinhonha e Mucuri
MSc Department of Dentistry Universidade Federal dos Vales Jequitinhonha e Mucuri
João Luiz de Miranda, Universidade Federal dos Vales Jequitinhonha e Mucuri
PhD Departamento of Basic Sciences Pathology Laboratory Universidade Federal dos Vales Jequitinhonha e Mucuri
Sandra Aparecida Marinho, State University of Paraíba
PhD Department of Dentistry State University of Paraiba Araruna Paraiba Brazil
Flaviana Dornela Verli, Universidade Federal dos Vales Jequitinhonha e Mucuri
Departamento of Basic Sciences Pathology Laboratory Universidade Federal dos Vales Jequitinhonha e Mucuri
Published
2016-11-01
Section
Original Research